Histone H1, the forgotten histone

Histone H1 binding is inhibited by histone variant H3.3.

Linker histones are involved in the formation of higher-order chromatin structure and the regulation of specific genes, yet it remains unclear what their principal binding determinants are. We generated a genome-wide high-resolution binding map for linker histone H1 in Drosophila cells, using DamID. H1 binds at similar levels across much of the genome, both in classic euchromatin and heterochro...

Binding of phosphorylated histone H1 to DNA.

A chromatin associated protein kinase was used to add 3 moles of phosphate to seryl side chains of 1 mole of histone H1. The DNA binding properties of this in vitro phosphorylated H1 were compared with those of unmodified H1. Considerably more radioactive superhelical DNA was retained on nitrocellulose filters at 20mM-40mM NaCl by phosphorylated H1 than by unmodified H1. However, zone velocity ...

Histone H1 gets Pin’d onto chromatin

Histone H1 gets Pin'd onto chromatin T he proline isomerase Pin1 limits chromosome decon-densation by stabilizing a histone's association with chromatin, Raghuram et al. reveal. Histone H1 incorporates into nucleosomes to enhance chromatin folding and condensation. Phosphory-lation by the cyclin-dependent kinase Cdk2 promotes H1's dissociation from chromatin, perhaps by altering the conformatio...

Reassociation of histone H1 to H1-depleted polynucleosomes.

A new procedure is described for the reassociation of histone H1 to rat liver polynucleosomes selectively depleted of H1 and nonhistone proteins. The fidelity of reconstitution was scrutinized by nuclease digestion and sedimentation studies monitoring the regeneration of structural features which disappear following removal of H1. The results demonstrate that the 166-base pair barrier of digest...

Nucleosome in Complex with Linker Histone H1